DNA Synthesis Custom Oligonucleotide Synthesis and Modification Service DNA SYNTHESIS & MODIFICATION FASMAC Oligonucleotide can be customized and ordered in various modification at 5′ and 3′ (terminal▼) and other modification sites (misc▼.). Fasmac Oligo Modification (terminal) Modification Molecular Weight & Chemical Structure Fluorescence Properties Purification 5′-end 3′-end Absorbance Max Wavelength Emission Max Wavelength RPC HPLC Amino(Standard) 266.16 209.18 Amino(C3) 137.08 137.08 Amino(C12) 263.32 Biotin 405.45 569.61 Phosphate 79.98 – 79.98 – FITC 655.55 568.57 495 nm 521 nm FAM 537.46 569.46 495 nm 521 nm TET 675.24 519 nm 539 nm HEX 744.13 537 nm 556 nm ROX (Rhodamine Red X) 786.16 – 699.18 – 587 nm 599 nm RITC 754.26 667.28 – Cy3 507.59 547 nm 563 nm Cy3.5 607.70 591 nm 604 nm Cy5 533.63 646 nm 662 nm Cy5.5 633.74 688 nm 707 nm TEXAS RED 853.86 766.88 – 596 nm 615 nm JOE 491.34 – 670.52 – 520 nm 548 nm TAMRA 695.62 – 623.60 556 nm 580 nm BHQ-1 554.49 534 nm – BHQ-2 556.47 579 nm – BHQ-3 597.63 672 nm – Dabcyl 521.54 462.44 DIG 811.94 724.96 SH 328.20 244.27 Cholesteryl 682.89 755.97 Stearyl 332.46 BIOTIN-TEG 569.61 – inverted dA 313.21 inverted dC 289.18 inverted dG 329.21 inverted dT 304.2 Fasmac Oligo Modification (miscellanious) Modification Molecular Weight & Chemical Structure Fluorescence Properties Purification Absorbance Max Wavelength Emission Max Wavelength RPC HPLC dI(Inosine) 314.19 dU(Deoxyuridine) 290.17 Phosphorthioate 16 5-Methyl-dC 303.21 I(RNA) 330.19 Spacer C3 138.06 Spacer 9 212.14 Spacer C12 264.30 Spacer 18 344.30 dSpacer 180.10 Amino-Modifier C2 dT 402.30 Amino-Modifier C6 dT 458.41 Amino-Modifier C6 dC 457.42 BHQ-1-dT 960.93 – 534 nm – BHQ-2-dT 962.91 – 579 nm – 5-Hydroxymethyl-dC 319.21 5-Hydroxymethyl-dU 320.19 Dabcyl-dT 709.00 476 nm – Fluorescein-dT 815.71 494 nm 522 nm dP 330.23 dK 358.25