Bacterial fruit blotch (BFB)

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Services outline	GMO	Germination Purity	Seedborne and other plant pathogens

Plant Pathology Test
Bacterial fruit blotch (BFB)

Submitted samples are divided into subsamples for below tests. Whole amount of samples are exhaustively used.

1) Culturing: Sweat-bag Seedling Method (Japanese Patent No.4633645; National Center for Seeds and Seedlings)
2) Genetic analysis: Endpoint PCR **Aac specific primer Realtime PCR **Aac / Aaa specific primer (Joint R&D with Tokushima University method) RealTime PCR **Aac specific primer (Modified ISHI method)
3） Medium test: AacSM selective medium
4） Inoculation test

Samples: Cucurbitaceae seeds
Small seed: Citrullus lanatus, Cucumis melo, Cucumis sativus etc.
Large seed: Cucurbita spp, Benincasa hispida, Momordica charantia var. pavel,
Lagenaria siceraria etc.

*Please contact us about the request for Plant Tissue.

Recommended Amounts of Seeds ： at least 10,000 or 30,000 seeds /lot

*Please contact us below if you request increase or decrease of submitting samples.

Inspection Items
I Endpoint PCR (Genetic Analysis) II Realtime PCR (Genetic Analysis)
III Endpoint PCR + Selective medium IV Realtime PCR + Selective medium
V Endpoint PCR + Selective medium + Inoculation test VI Realtime PCR + Selective medium + Inoculation test

For more information about the price and schedule, please contact us below.

Prior to submitting a sample, please ask for the Sample Submission Forms. Please fill it out and enclose it with the sample.

FASMAC CO., LTD.

Business Development Unit (Seed and Seedling Testing Team)