Bacterial fruit blotch (BFB)
Submitted samples are divided into subsamples for below tests. Whole amount of samples are exhaustively used.
- 1) Culturing :
Sweat-bag Seedling Method(Japanese Patent No.4633645; National Center for Seeds and Seedlings)
- 2) Genetic analysis :
Endpoint PCR **Aac specific primerRealtime PCR **Aac / Aaa specific primer(Joint R&D with Tokushima University method)RealTime PCR **Aac specific primer (Modified ISHI method)
- 3) Medium test :
- AacSM selective medium
- 4) Inoculation test
Test Samples and Recommended Amounts of Samples
- Samples :
- Cucurbitaceae seeds
- Small seed :
Citrullus lanatus, Cucumis melo,Cucumis sativus etc.
- Large seed :
Cucurbita spp, Benincasa hispida,Momordica charantia var. pavel, Lagenaria siceraria etc.
Recommended Amounts of Seeds ： at least 10,000 or 30,000 seeds /lot
*Please contact us below if you request increase or decrease of submitting samples.
*Please contact us about the request for Plant Tissue.
- I.Endpoint PCR (Genetic Analysis)
- III.Endpoint PCR + Selective medium
- V.Endpoint PCR + Selective medium + Inoculation test
For more information about the price and schedule, please contact us below.
How to Submit a Sample
Prior to submitting a sample, please ask for the Sample Submission Forms. Please fill it out and enclose it with the sample.
FASMAC CO., LTD.
Business Development Unit (Seed and Seedling Testing Team)
E-mail : firstname.lastname@example.org