Bacterial fruit blotch (BFB)

Submitted samples are divided into subsamples for below tests. Whole amount of samples are exhaustively used.

1) Culturing　　　　 :

Sweat-bag Seedling Method

(Japanese Patent No.4633645; National Center for Seeds and Seedlings)

2) Genetic analysis　:

Endpoint PCR **Aac specific primer

Realtime PCR **Aac / Aaa specific primer

(Joint R&D with Tokushima University method)

RealTime PCR **Aac specific primer (Modified ISHI method)

3) Medium test　　　:

AacSM selective medium

4) Inoculation test

Samples　　　 :

Cucurbitaceae seeds

Small seed　　 :

Citrullus lanatus, Cucumis melo,

Cucumis sativus etc.

Large seed　　 :

Cucurbita spp, Benincasa hispida,

Momordica charantia var. pavel, Lagenaria siceraria etc.

1. I.Endpoint PCR (Genetic Analysis)
2. II.Realtime PCR (Genetic Analysis)
3. III.Endpoint PCR + Selective medium
4. IV.Realtime PCR + Selective medium
5. V.Endpoint PCR + Selective medium + Inoculation test
6. VI.Realtime PCR + Selective medium + Inoculation test